HPLC Column, RP C8 Octyl, Wide Pore, 5um, 4.6mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C8™ Wide Pore HPLC Columns are designed for reliable reversed-phase separations of larger molecules that require increased pore accessibility. The stationary phase consists of a traditional octyl (C8) bonded ligand permanently attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while providing consistent retention and excellent peak shape. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to fully access the bonded surface for improved chromatographic performance.
68508-05P-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 50 mm length format. The short 50 mm column length is optimized for rapid analyses while maintaining excellent chromatographic efficiency. The 300 Å pore architecture is particularly beneficial for proteins, peptides, biomolecules, and long-chain fatty acids that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than a comparable C18 phase while maintaining excellent selectivity for larger non-polar compounds.
This column size is widely used for high-throughput screening, rapid method development, and routine analytical applications. The 4.6 mm ID format operates efficiently on conventional HPLC systems and typically performs best at flow rates of approximately 1.0-2.0 mL/min. The combination of short run times, good resolution, and broad instrument compatibility makes this one of the most popular column configurations for analytical laboratories.
Key Benefits
RP C8™ Wide Pore stationary phase
Octyl (C8) bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
Ideal for proteins and peptides
Lower retention than C18 phases
Fast 50 mm column length
Low backpressure with 5 µm particles
Excellent for high-throughput screening
Typical Applications
Reversed-phase HPLC
High-throughput screening
Protein analysis
Peptide analysis
Biomolecule separations
Long-chain fatty acid analysis
Pharmaceutical analysis
Biopharmaceutical analysis
Method development
Routine quality control testing
Specifications
Product Type: HPLC Column
Stationary Phase: RP C8™ Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Octyl (C8)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 4.6 mm ID x 50 mm
Recommended Flow Rate: 1.0-2.0 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Designed for larger molecule reversed-phase separations
300 Å pores enhance accessibility for proteins, peptides, and biomolecules
Provides lower retention than comparable C18 columns
Suitable for hydrophobic and moderately non-polar compounds
Excellent choice for long-chain fatty acids and peptide analyses
50 mm length helps reduce analysis times and solvent consumption
5 µm particles provide low backpressure and broad instrument compatibility
Operates efficiently on standard HPLC systems
Frequently selected for high-throughput screening applications
Manufactured using ultra-high purity silica and fully double end capped bonding technology